Beta-cell conversion
(A) Representative pancreatic sections from 3-month-old mouse Dnmt1fl/fl (control) and RIP-Cre:Dnmt1fl/fl (RC:Dnmt1fl/fl) littermates were immunostained for glucagon
(Glu; green) and Dnmt1 (red).
(B) Immunostaining of representative pancreatic sections from 8-month-old Dnmt1fl/fl and RC:Dnmt1fl/fl littermates showing insulin (green), glucagon (red), and overlay with DAPI (to counterstain the nuclei; blue).
(C) Quantification of glucagon and insulin area in 8-month-old RIP-Cre:Dnmt1fl/+ (control) and RIP-Cre:Dnmt1fl/fl animals, shown as percentage of total islet area (n = 4 animals). The error bars represent SEM (***p < 0.005).
(Image credit: Sangeeta Dhawan, Senta Georgia/UCLA)
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